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000 × g for 20 min at 4 °C and the resulting pellet was washed twice with 0.1 M ammonium acetate in 100 % methanol, 80 % ice-cold acetone, and once in 70 % ethanol by centrifugation at 20,000 × g for 20 min at 4 °C. After the final wash, supernatant was decanted and the protein pellet was dried at 37 °C for not more than 15 min followed by solubilization with lysis buffer [7 M urea, 2 M thiourea, 4 % CHAPS, 18 mM Tris-HCl (pH 8.0), 14 mM Trizma base, and two EDTA-free proteinase inhibitor cocktail tablets (Roche Diagnostics GmbH, Baden-Württemberg, Germany) in a final volume of 100 ml buffer, 0.2 % Triton X-100 (R), containing 50 mM DTT]. Protein lysates were centrifuged at 20,000 × g for 20 min at 4 °C and the resulting supernatant was stored in aliquots at -80 °C. Protein concentration was determined using 2-D Quant Kit according to manufacturer's standard procedure.. Some limitations of this study have to be addressed. First, the assessment of SVV with FloTrac/Vigileo system has not yet been proven by direct comparison with other techniques. However, our findings are strongly supported by some studies in which SVV was assessed with PiCCO system and found to be a favorable predictor of fluid responsiveness [9-11, 41]. Second, our patients were hypovolemic at baseline because of pre-operative fasting, gastrointestinal preparations and application of general anesthetics. The predictive value of SVV was not evaluated in normovolemic or even hypervolemic patients with SVV within or below the normal range. However, it seems likely that the present setting is close to most common clinical situations, with hypovolemia as the major cause of arterial hypotension. Moreover, during measurements and fluid trial, manipulations were not allowed, ie, table-tilting maneuvers, catheter insertion, or surgical interventions were strictly avoided. These conditions rarely apply to clinical situations, when the decision on fluid replacement therapy has to be made.. Recognition of HLA alleles is useful in transplantation and in anthropological and disease studies. Acute lymphoblastic leukemia (ALL) is the most common blood cancer. It is now generally agreed that both genetic and environmental factors play an interactive role in the development of ALL disease. It is unknown whether there exists a restriction to certain MHC genotypes in leukemia like ALL.. ER stress response as a synergist target for oncolytic virus therapy by. PI polyamides targeting 8-bp sequences at the cellular level. Polyamides. mechanism by which formalin induces DNA denaturation has been.
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Moreover, rasburicase should be not administrated in pregnancy.. to break down alcohol mean that. from an increase in bone resorption relative to bone formation. In.
Talk given for the symposium Humanities Beyond Disciplines, organized by the Department of English and IQAC, Sammilani Mahavidyalaya, Kolkata, India on 7/24/2020. View at: https://youtu.be/NlM1GIzXzSg
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This illustrated talk introduces the history of Indian wisdom traditions from its beginning in the Indus Valley (c. 2700 BCE) to its continued development into modernity. It touches on the Vedas, the Upanishads, Buddhism, Sankhya and Yoga, Bhagavad Gita and Bhagavatism , Shaivism and Shaktism, discussed in the context of Indian social history. Coming to …
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A Big Turtle Show conversation hosted by Vikram Zutshi and Lea Horvatic in June 2020.
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Talk given to students of Dr. Nalini Rao at SOKA University, Mission Viejo, Orange County, CA.
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Interview by Suma Gowda, Jonathan Kay and Alicia Gonzales apropos the article Living Laboratories of the Life Divine being published by Collaboration the journal of the Sri Aurobindo Society as part of its "Gems from the Archive" - December 2020
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Interview on Posthumans V-log by Francesca Ferrando, leading philosopher of philosophical posthumanism - April 2020
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Revised version of a talk titled "Hindu-Islamic Mysticism in Three Pioneers of Indian Neo-Tantric Art: S. H. Raza, G. R. Santosh, and Sohan Qadri" given at the Graduate Theological Union's Doug Adams gallery on 2/27/2020
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